Multiplex BPV Genotyping

The offered service is designed to simultaneously detect and genotype 

• Bovine Papilomavirus 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and BaPV11

Test specifications

Genotyping of BPV is done after DNA extraction with specific primer sequences in a multiplex PCR targeting the viral L1 gene, and subsequent hybridisation using specific oligonucleotide probes. For control of DNA quality the presence of the PolA gene is tested as a reference. Several positive and negative controls are included to test PCR performance.

Procedure

To be done by the user

• DNA isolation or lysate preparation according to the BPV lysate protocol
• Sign in and complete the BPV Genotyping Order Form
• Label tubes with your name and a specific sample name as provided in the Order Form

To be done by Multiplexion

• Multiplex PCR-based amplification of BPV
• Detection and Genotyping of PCR products by specific oligonucleotide probes
• Transfer of results to customer by Email or Fax
  

Report - Confidentiality Notice

• all customer information is treated as strictly confidential
• formal, contractual confidentiality agreements can be generated upon request
• reports are maintained in our archives for two years; samples are kept up to six months for retesting at customers' request
  

Sample Preparation

Please find a detailed description of the sample preparation on the sample submission form.

Shipping Instructions

The samples can be sent to our laboratory without an announcement. A complete copy of our sample submission form must be enclosed with all submitted samples.

Prizes

Please contact us for your offer. Special prizes for universities and institutes, and bulk prizes available. 

Please note: This service is designed to help you comply with safety regulations, to improve quality control and to help your research. It is NOT suited for clinical diagnostics.

For more information, please contact us.

Multiplexion - Advanced Detection