Multiplex Human Herpesvirus (HHV) Genotyping

The offered service is designed to simultaneously detect and genotype 

• Human Herpes simplex virus HSV1 and HSV2, varicella zoster virus (VZV), Epstein-Barr-Virus (EBV), cytomegalovirus (CMV), HHV6, HHV7, and HHV8.

Test specifications

Genotyping of HHV is done after DNA extraction with specific primer sequences in a multiplex PCR, and subsequent hybridisation using specific oligonucleotide probes. For control of DNA quality the presence of the PolA gene is tested as a reference. Several positive and negative controls are included to test PCR performance.

Procedure

To be done by the user

• DNA isolation from appropriate clinical specimens such as bronchoalveolar lavage, conjunctival fluid, sore secretion, blister material, plasma, serum and urine
• Sign in and complete the HHV Genotyping Order Form
• Label tubes with your name and a specific sample name as provided in the Order Form

To be done by Multiplexion

• Multiplex PCR-based amplification of HHV
• Detection and Genotyping of PCR products by specific oligonucleotide probes
• Transfer of results to customer by Email or Fax
  

Report - Confidentiality Notice

• all customer information is treated as strictly confidential
• formal, contractual confidentiality agreements can be generated upon request
• reports are maintained in our archives for two years; samples are kept up to six months for retesting at customers' request
  

Sample Preparation

Please send us purified DNA (50 ng of genomic DNA) isolated from appropriate clinical specimens such as bronchoalveolar lavage, conjunctival fluid, sore secretion, blister material, plasma, serum and urine.

Shipping Instructions

The samples can be sent to our laboratory without an announcement. A complete copy of our sample submission form must be enclosed with all submitted samples.

Prizes

Please contact us for your offer. Special prizes for universities and institutes, and bulk prizes available. 

Please note: This service is designed to help you comply with safety regulations, to improve quality control and to help your research. It is NOT suited for clinical diagnostics.

For more information, please contact us.

Multiplexion - Advanced Detection