Authentication of human cell lines by STR profiling


  • For cell lines not included in our SNP typing data base, we recommend human cell line authentication using STR profiling
  • Analysis of the following markers: CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX, vWA, and the gender marker Amelogenin
  • Includes all relevant markers of ANSI/ATCC ASN0002-2011. Authentication of human cell lines: Standardization of STR profiling
  • Authentication of newly-established human cell lines or sublines by genotyping paired donor and derived cell line samples


To be done by the user

  • Purify cell line DNA by commercially available DNA purification systems, e.g. from Qiagen or Roche (protocol)
    • required DNA concentration: 15 ng/µL
    • required amount: >40 µL
    • tubes: 1.5 ml SafeLock tubes (please do not use screw caps and Parafilm)
  • Complete the
  • Label sample tubes with "STR" and the specific sample name as provided in the confirmation Email
  • Ship samples in a padded envelope WITH a copy of the confirmation Email to: 

 Multiplexion, c/o DKFZ, Dept. F022, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany


To be done by Multiplexion

  • STR profiling of submitted cell line(s)
  • Comparison of obtained signature to reference signatures at DSMZ, ATCC (optionally).
  • Chromatogram of the DNA profile as PDF (optionally).
  • Transfer of results to customer via Email (the report can be used as official certificate for granting agencies and peer-reviewed journals)


Turnaround Time

Usually 5-10 working days upon receipt of samples.

  • Until February 2020: Customers from Heidelberg can drop off samples in the building INF242 between 9 AM and 12 AM each Monday.
  • Starting from March 2020: Customers from Heidelberg can drop off samples in the building INF242 between 9 AM and 12 AM each Friday.


What to do with contaminated cell lines?

  • Different contaminations require different actions. An overview can be found here.
  • A database of misidentified or cross-contaminated cell lines can be found here. Please stop working with any of these cell lines.
  • Problems with contaminations? Our check list for sterile work may help.


Please note: This service is FOR RESEARCH USE ONLY. Do not use in diagnostic procedures.

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