Authentication of murine cell lines by STR profiling

 

  • For murine cell lines, we recommend cell line authentication using STR profiling
  • Analysis of the following markers: 18-3, 4-2, 6-7, 9-2, 15-3, 6-4, 12-1, 5-5, X-1 nach Almeida et al. (2014) Mouse cell line authentication. Cytotechnology 66: 133-147
  • Authentication of newly-established cell lines or sublines by genotyping paired donor and derived cell line samples
  • Data base screen not possible at the moment due to lack of publicly available murine cell line data base

 

To be done by the user

  • Purify cell line DNA by commercially available DNA purification systems, e.g. from Qiagen or Roche (protocol)
    • required DNA concentration: 15 ng/µL
    • required amount: >40 µL
    • tubes: 1.5 ml SafeLock tubes (please do not use screw caps and Parafilm)
  • Complete the
  • Label sample tubes with "Mouse-STR" and the specific sample name as provided in the confirmation Email
  • Ship samples in a padded envelope WITH a copy of the confirmation Email to: 

 Multiplexion, c/o DKFZ, Dept. F022, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany

 

To be done by Multiplexion

  • STR profiling of submitted cell line(s)
  • Chromatogram of the DNA profile as PDF (optionally).
  • Transfer of results to customer via Email (the report can be used as official certificate for granting agencies and peer-reviewed journals)

 

Turnaround Time

Usually 5-10 working days upon receipt of samples.

  • Customers from Heidelberg can drop off samples in the building INF242 between 9 AM and 12 AM on Monday.

 

What to do with contaminated cell lines?

  • Different contaminations require different actions. An overview can be found here.
  • A database of misidentified or cross-contaminated cell lines can be found here. Please stop working with any of these cell lines.
  • Problems with contaminations? Our check list for sterile work may help.

 

Please note: This service is FOR RESEARCH USE ONLY. Do not use in diagnostic procedures.

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